Every readytouse ladder is supplied with the nuclease free loading dye solution, which ensures optimal migration and quantification of your dna probes. For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder. Do not heat the trackit 1kb plus dna ladder before loading. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from. All rna molecules in the ladder are polyatailed 40 nt for convenient. Download a pdf containing pricing for our full product list. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. The ladder consists of nine bands in even increments 0.
Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of. Use uv transilluminator to detect dna bands stained with ethidium bromide. The key to accurate band analysis is to use the correct dna ladder for your particular application. Nebs quickload purple dna ladders offer sharp, uniform bands with easily identifiable reference bands.
The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. The 1 kb and 3 kb bands contain more dna to provide internal orientation. This ladder has 12 evenly spaced bands ranging from 1kb. All include a vial of gel loading dye, purple 6x, no sds. There have been no changes to product formulation or specifications. First aid measures general information this solution is not hazardous. Ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650 base pairs that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder.
The product is supplied in a solution of 10 mm trishcl, ph 8. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Thermo scientific pierce 1step transfer buffer is designed. View more information about our dna stains technology. Sigmaaldrich offers a number of directload 1 kb dna ladder products. The 1 kb plus dna ladder for safe stains is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. All of these form less than 1 % wv of the final mixture, so they are not listed in the table above. Every readytouse ladder is supplied with the nucleasefree loading dye solution, which ensures optimal migration and quantification of your dna probes. The 1 kb dna ladder contains discrete dna fragments ranging from 300 bp to. Use the invitrogen dna ladder selection table below to help you choose the dna ladder you. The 50bp sizemarker dnaladder from geneon is containing orange g. How can i prepare working solution with 1 kb dna ladder. The dna ladder consists of proprietary plasmids which.
One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Invitrogen trackit 1 kb plus dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Trackit 1 kb plus dna ladder thermo fisher scientific. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied.
Invitrogen 1kb dna extension ladder 100ug nucleic acid. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. These are available online in convenient and compact pdf format. The egel 1kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100 base pairs to 12,000 base pairs. In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp. The 1,000bp and 3,000bp fragments have increased intensity relative to the other. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination.
Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. Use blue light transilluminator to detect dna bands stained with sybr stains.
Catalog number size important guidelines 10787018 250 g. The ladder is supplied in gel loading buffer and can be applied directly to the gel. The bands of the ladder each contain from 1 to 12 repeats of. If samples need to be diluted, use te or other buffer of minimal ionic strength. Tools to accelerate your life science research fisher scientific. Dear customer, geneon likes to send free samples to convince the valued. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb.
The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple. Readyload 1 kb plus dna ladder is intended for use. All of these form less than 1 % wv of the final mixture, so they are. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. Brightmax 50010 kb dna ladder is a precise and highly stable dna. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Readyload 1 kb plus dna ladder is intended for use as a molecular weight standard in agarose and polyacrylamide gel electrophoresis. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Thaw, mix and briefly centrifuge each component before use. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. To create our dna ladders, each fragment is purified individually using proprietary chromatographybased technology, resulting in dna ladders of superior quality which exceeds industry standards. This product is available in quickload purple format enjoy the convenience of a readytoload format.
Invitrogen dna ladders are available for a wide variety of size ranges, applications, and formats. Gibco psc neural induction medium is a serumfree medium. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. Invitrogen egel 1 kb plus dna ladder 100 applications. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp.
The ladder consists of bands that are generated from pcr and restriction enzyme. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. A rapid and economic inhouse dna purification method using. Powerful tool for estimating molecular weight of linear, doublestranded dna fragments.
This marker is ideal for the size determination of digested dna. All rna molecules in the ladder are polyatailed 40 nt for convenient use as control templates for cdna synthesis. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. Neb dna ladders are available in standard formats and in a variety of convenient, readytoload formats. This format is available for the low molecular weight, 50 bp, 100 bp and 1 kb plus dna ladders. Readyload 1 kb p dna ladder thermo fisher scientific. The dna ladder preparation contains, amongst other components, 2 mm trishcl ph 7. Generuler 1 kb dna ladder thermo fisher scientific. Dna ladder 50 bp readyto use dna ladder geneonbioscience. The 1 kb plus dna ladder for safe stains might be used on sybr green i precast agarose gels but might require further dilution and optimization. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. The 1 kb dna ladder is a readytoload molecular weight marker suitable for. Powerful tool for estimating molecular weight of linear, doublestranded.